Figure 2.4.32.4.3 Hypothetical fluctuation of scattering intensity of larger particles and smaller particles. Figure 2.4.32.4.3 shows the hypothetical fluctuation of scattering intensity of larger particles and smaller particles. Then, as the rate of these intensity fluctuations depends on how fast the particles are moving (smaller particles diffuse faster), information about the size distribution of particles in the solution could be acquired by processing the fluctuations of the intensity of scattered light. Since the distance between particles also affects the phase overlap/interfering of the diffracted light, the brightness and darkness of the spots in the “speckle” pattern will in turn fluctuate in intensity as a function of time when the particles change position with respect to each other. Importantly, for a system undergoing Brownian motion, small particles should diffuse faster than large ones.(Δx)2¯¯¯¯¯¯¯¯¯¯¯¯¯ = 2Δt(2.4.2)(2.4.2)(Δx)2¯ = 2ΔtD =kBT6πμa(2.4.3)(2.4.3)D =kBT6πμaĪs a result of the Brownian motion, the distance between particles is constantly changing and this results in a Doppler shift between the frequency of the incident light and the frequency of the scattered light. In practice, particle samples are normally not stationary but moving randomly due to collisions with solvent molecules as described by the Brownian motion, 2.4.22.4.2, where (Δx)2¯¯¯¯¯¯¯¯¯¯¯¯¯(Δx)2¯ is the mean squared displacement in time t, and D is the diffusion constant, which is related to the hydrodynamic radius a of the particle according to the Stokes-Einstein equation, 2.4.32.4.3 , where k B is Boltzmann constant, T is the temperature, and μ is viscosity of the solution. Image used with permission (Public Domain Epzcaw). This is the light field formed when a laser beam was scattered from a plastic surface onto a wall. A photograph of an objective speckle pattern. Figure 2.4.22.4.2 Typical speckle pattern. If that diffracted light is projected as an image onto a screen, it will generate a “speckle” pattern (Figure 2.4.22.4.2 ) the dark areas represent regions where the diffracted light from the particles arrives out of phase interfering destructively and the bright area represent regions where the diffracted light arrives in phase interfering constructively. Figure 2.4.12.4.1: Scheme of Rayleigh scattering. According to the Rayleigh scattering (Figure 2.4.12.4.1), when a sample of particles with diameter smaller than the wavelength of the incident light, each particle will diffract the incident light in all directions, while the intensity II is determined by 2.4.12.4.1 , where I0I0 and λλ is the intensity and wavelength of the unpolarized incident light, RR is the distance to the particle, θθ is the scattering angel, nnis the refractive index of the particle, and rr is the radius of the particle. The theory of DLS can be introduced utilizing a model system of spherical particles in solution. Finally, data analysis of the DLS measurement, and the applications of DLS as well as the comparison against other size-determine techniques will be shown and summarized. Herein a brief introduction of basic theories of DLS will be demonstrated, followed by descriptions and guidance on the instrument itself and the sample preparation and measurement process. Since the scattering pattern of the light is highly correlated to the size distribution of the analyzed particles, the size-related information of the sample could be then acquired by mathematically processing the spectral characteristics of the scattered light.

In the DLS experiment, normally a laser provides the monochromatic incident light, which impinges onto a solution with small particles in Brownian motion.Īnd then through the Rayleigh scattering process, particles whose sizes are sufficiently small compared to the wavelength of the incident light will diffract the incident light in all direction with different wavelengths and intensities as a function of time. Click here to see more posts about zeta potential/DLS Only 8$ for interpretation of your DLS resultsĭynamic light scattering (DLS), which is also known as photon correlation spectroscopy (PCS) or quasi-elastic light scattering (QLS), is a spectroscopy method used in the fields of chemistry, biochemistry, and physics to determine the size distribution of particles (polymers, proteins, colloids, etc.) in solution or suspension.

Dynamic light scatter